Our primary goal was to develop two real-time multiplex polymerase chain reaction (PCR) assays for simultaneous detection of ...

Varicella-zoster virus causes chickenpox and is a member of the herpes virus group. It is extremely contagious and causes a ...

Dietary protein absorption in neonatal mammals and fishes relies on the function of a specialized and conserved population of highly absorptive lysosome rich enterocytes (LREs). The gut microbiome has ...

DNA concentration and purity was monitored on 1% agarose gels. The V3–V4 region of the bacterial 16S rRNA gene was PCR amplified using the specific primers 341F (5′-CCTAYGGGRBGCASCAG-3′) and 806R ...

The quality of digital PCR data can only be as high as the assays used to obtain it. High-quality assays ensure that the ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

The GenDx NGSgo-X11-3 handbook states that the PCR master mix and amplification primers for each long-range PCR mix were combined. Each mix (A, B, and C) amplifies various HLA genes (Table 1 ...

This important study explores the interplay between gene dosage and gene mutations in the evolution of antibiotic resistance. The authors provide solid evidence to connect proteostasis with gene ...

These first microbes would have been extremely simple. Meanwhile, viruses degrade more easily than bacteria, so there are no physical fossils of viruses. Related: What was the first animal on Earth?

Researchers have engineered bacteria as personalized cancer vaccines that activate the immune system to specifically seek out and destroy cancer cells. Columbia researchers have engineered ...